Fig. 3: CDH12 is upregulated in anastatic breast cancer cells. | Oncogenesis

Fig. 3: CDH12 is upregulated in anastatic breast cancer cells.

From: Chemotherapy-induced executioner caspase activation increases breast cancer malignancy through epigenetic de-repression of CDH12

Fig. 3

A PCA of the RNA sequencing data of ADR-ZsGreen+ and ADR-ZsGreen− cells derived from BT474 cells. B Volcano plot showing genes differentially expressed in BT474-ADR-ZsGreen+ and BT474-ADR-ZsGreen− cells. C GO enrichment of genes upregulated in BT474-ADR-ZsGreen+ cells. D PCA of the RNA sequencing data of ADR-ZsGreen+ and ADR-ZsGreen− cells derived from MDA-MB-231 cells. E Volcano plot showing genes differentially expressed in MDA-MB-231-ADR-ZsGreen+ and MDA-MB-231-ADR-ZsGreen− cells. F GO enrichment of genes upregulated in MDA-MB-231-ADR-ZsGreen+ cells. G On the top is the Venn diagram showing genes that were commonly upregulated more than 4 folds in the ADR-ZsGreen+ cells derived from BT474 cells and MDA-MB-231 cells. The bottom is the heat map illustrating the expression level of the 16 commonly upregulated genes in each sample. H qRT-PCR results of the GDAP1L1, INHBE and CDH12 in the ZsGreen+, the ZsGreen− and the control cell populations derived from BT474 (top) or MDA-MB-231 (bottom) cells. n = 3. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA with Tukey test. *P < 0.05; **P < 0.01; ***P < 0.001. I Western blots of CDH12 in the indicated cell populations. J Kaplan–Meier plots showing that patients with high CDH12 expression had significantly worse progression-free survival than those with low CDH12 expression. Left: analysis result of GSE20685. Right: analysis result of GSE69031.

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