Fig. 5: CDH12 promotes breast cancer cell proliferation and migration via ERK-CREB signaling. | Oncogenesis

Fig. 5: CDH12 promotes breast cancer cell proliferation and migration via ERK-CREB signaling.

From: Chemotherapy-induced executioner caspase activation increases breast cancer malignancy through epigenetic de-repression of CDH12

Fig. 5

A, B GO enrichment analysis of genes downregulated after knocking down CDH12. C Western blots showing the effect of CDH12 knockdown on the protein levels of CDH12, phosphorylated ERK (p-ERK) and ERK proteins. D Western blots showing the effect of ERK knockdown on CDH12, p-ERK, ERK, phosphorylated CREB (p-CREB) and CREB proteins. E The effect of ERK knockdown on EdU incorporation of the indicated cell populations. n = 3. F The effect of ERK knockdown on transwell migration of the indicated cell populations. n = 3. Scale bar: 200 μm. G Western blots showing the effect of knocking down CDH12 on the protein levels of CDH12, p-CREB, CREB in the indicated cell populations. H Western blots showing the effect of CREB knockdown on the protein levels of CDH12, p-CREB, CREB. I The effect of CREB knockdown on EdU incorporation in the indicated cell populations. n = 3. J The effect of CREB knockdown on transwell migration of the indicated cell populations. n = 3. Scale bar: 200μm. Data are presented as mean ± SEM. Statistical significance was determined using one-way ANOVA with Tukey test. *P < 0.05; **P < 0.01; ***P < 0.001.

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