Fig. 2: Knockdown of AIFM2 suppressed metastasis of HCC cells in vitro and in vivo. | Oncogenesis

Fig. 2: Knockdown of AIFM2 suppressed metastasis of HCC cells in vitro and in vivo.

From: AIFM2 promotes hepatocellular carcinoma metastasis by enhancing mitochondrial biogenesis through activation of SIRT1/PGC-1α signaling

Fig. 2

A and B Downregulation of AIFM2 was tested in SNU-423 and HLF cells upon transfection with siRNAs targeting AIFM2 by qRT-PCR and western blot analysis. C and D Short- and long-term cell proliferation was determined by MTS cell viability (C) and colony formation (D) assays in SNU-423 and HLF cells upon transfection with siRNAs targeting AIFM2. E and F Cell apoptosis and cell cycle were analyzed by flow cytometry in SNU-423 and HLF cells upon transfection with siRNAs targeting AIFM2. G and H Transwell migration and invasion assays in SNU-423 and HLF cells upon transfection with siRNAs targeting AIFM2. I Number of lung metastases was evaluated by Hematoxylin-Eosin staining in nude mice injected with AIFM2 knockdown or control SNU-423 cells (6 mice per group). Scale bars, 20 μm.

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