Fig. 3: B4GALT1 regulates HCC cell metastasis in vivo. | Oncogenesis

Fig. 3: B4GALT1 regulates HCC cell metastasis in vivo.

From: Decreased B4GALT1 promotes hepatocellular carcinoma cell invasiveness by regulating the laminin-integrin pathway

Fig. 3

A Western blots showing B4GALT1 knockout (ko-B4GALT1-1 and ko-B4GALT1-2) in PLC5 cells, and B4GALT1 overexpression (B4GALT1) in HA22T cells. B Effects of B4GALT1 knockout on the migration and invasion of PLC5 cells analyzed via transwell migration and Matrigel invasion assays, respectively. Representative results from three independent experiments are shown. C Effects of B4GALT1 knockout on tumor metastasis. Representative images of lung metastasis in parental and B4GALT1 knockout PLC5 cells are shown. The arrows indicate tumor nodules. Scale bar, 500 µm. PLC5 cells were intravenously injected into NOD/SCID mice (n = 7 per group) and after 60 days, the mice were sacrificed and their lungs were visualized. D Effects of B4GALT1 overexpression on tumor metastasis. Representative images of lung metastasis of HCC in mock and B4GALT1 overexpressing HA22T cells; n = 7 per group; one mouse in the mock control group died during the experiment. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01, Student’s t-test.

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