Fig. 2: KIF18B promotes HCC cell proliferation and migration. | Oncogenesis

Fig. 2: KIF18B promotes HCC cell proliferation and migration.

From: Kinesin family member 18B activates mTORC1 signaling via actin gamma 1 to promote the recurrence of human hepatocellular carcinoma

Fig. 2

A Expression of KIF18B in normal human hepatic cell line and HCC cell lines examined by western blot. B, C The influence of KIF18B silencing (B) and ectopic expression (C) on HCC cell proliferation determined by cell counting kit-8 (CCK-8) assay. D, E Colony formation images of KIF18B silencing (D) and KIF18B overexpression (E) HCC cells. The colonies were captured and counted. F The growth of HCC subcutaneous tumors is suppressed by KIF18B silencing. G Subcutaneous tumors were measured by volume and weight. H IHC analysis of the expression levels of KIF18B, Ki-67, and p-mTOR in the indicated subcutaneous tumors. Scale bars, 50 μm. I, K The influence of KIF18B silencing (I) and ectopic expression (K) on HCC cell migration determined by wound healing assay. Scale bars, 200 μm. J, L Transwell assay images of KIF18B silencing (J) and overexpression (L) HCC cells. The cells were captured and counted. Scale bars, 100 μm. Error bars indicate the mean ± SD of three independent experiments. P values were calculated by student t test, *P < 0.05, **P < 0.01, ***P < 0.001.

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