Fig. 1: Tiled sgRNAs identify a locus that critical for super-enhancer-induced PD-L1 expression. | Oncogenesis

Fig. 1: Tiled sgRNAs identify a locus that critical for super-enhancer-induced PD-L1 expression.

From: Identifying a locus in super-enhancer and its resident NFE2L1/MAFG as transcriptional factors that drive PD-L1 expression and immune evasion

Fig. 1: Tiled sgRNAs identify a locus that critical for super-enhancer-induced PD-L1 expression.

A Schematic representation of the genomic locations of CD274, CD273, as well as the super-enhancer PD-L1L2-SE, which was divided into 3 elements (C1, C2, C3); B Precise analysis of BRD4 binding region between JQ1 and DMSO treated cells from Chr9:5,496,378 to Chr9:5,499,663. BRD4 ChIP-sequencing data was downloaded from GEO database: GSM2330549 and GSM2330551; C The location of a 850 bp DNA region in PD-L1L2-SE super-enhancer and saturated design of all potential specific sgRNAs; D SUM-159 cells were stably transfected with indicated sgRNAs. Then western blotting analysis shows the protein level of PD-L1 in each stably genetic modified cell lines. E Real-time PCR was performed to analyze the mRNA level of PD-L1 and PD-L2 in sgVector and Sg-22 cells. F The surface expression of PD-L1 (PE) and PD-L2 (APC) in sgVector and sg-22 cells were determined by FACS. G Immunofluorescence was performed to analyze the distribution and expression of PD-L1 (Green) in sgVector and sg-22 cells. Hoechst was used as nuclear staining. Data in (D) and (G) are representative of two independent experiments. Data in (E) and (F) are representative of three independent experiments. *p < 0.05; **p < 0.01, ***p < 0.001.

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