Fig. 6: Pharmacological intervention of SMYD3 enhances the response of EC cells to radiotherapy. | Oncogenesis

Fig. 6: Pharmacological intervention of SMYD3 enhances the response of EC cells to radiotherapy.

From: SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair

Fig. 6: Pharmacological intervention of SMYD3 enhances the response of EC cells to radiotherapy.

A Flow cytometry analysis of GFP+ and DsRed+ cells of EJ5-U2OS cells upon BCI-121 treatment. B Western blotting of the indicated proteins in WCLs from HEC1B cells upon treatment with BCI-121. C CCK-8 cell proliferation analysis of HEC1B cells upon BCI-121 treatment. D Colony formation assay of HEC1B cells upon BCI-121 addition. E, F Left graph, cell migration analysis (E), and cell invasion analysis (F) of HEC1B cells upon BCI-121 treatment. Right graph, the quantitative data are shown as the mean ± SD (n = 3). Scale bar, 200 μm. G Images of HEC1B cells upon either BCI-121 treatment irradiation or combined treatments. H Images of the tumors from HEC1B cells treated with BCI-121 (500 μM) or irradiation or combined treatment. I The weight of xenograft tumors shown in G. n = 5. J The tumor volume of xenograft tumors was measured on the indicated days after injection. K Representative images of immunofluorescence staining of the expression of Ki67 and cleaved-Caspase3. Scale bar, 30 μm. L The quantitative IHC data shown in K.

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