Fig. 2: Interference with USP32 inhibits NSCLC cell proliferation and migration. | Oncogenesis

Fig. 2: Interference with USP32 inhibits NSCLC cell proliferation and migration.

From: USP32 facilitates non-small cell lung cancer progression via deubiquitinating BAG3 and activating RAF-MEK-ERK signaling pathway

Fig. 2

A, B Western blot was used to detect USP32 protein level after transfection of A549/H1299 cells with siUSP32#1 and siUSP32#2. C, D CCK-8 and plate cloning assays were performed to detect change in cell proliferation ability of A549/H1299 cells transfected with siUSP32#1 and siUSP32#2 compared to control. E, F Transwell and scratch assay were used to analyze the migratory capacity of A549/H1299 cells after interference with USP32. Scale bar:100 μm. G The levels of epithelial-mesenchymal transition markers (E-cadherin protein, Vimentin protein) were assessed using Western blotting after interference with USP32 in A549/H1299 cells; *p < 0.05; **p < 0.01; ***p < 0.001.

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