Fig. 2: Knockdown of DKK1 and CKAP4 inhibits AKT activity and cell growth in OR CRC cells.
From: DKK1 as a chemoresistant protein modulates oxaliplatin responses in colorectal cancer
A Representative western blots of SW48-OR cells stably expressing either a scrambled shRNA or shRNAs specific for DKK1 (left) and quantification (right). β-Actin served as a loading control. *p < 0.05, **p < 0.01. Data are mean ± SEM. (n = 3). B Representative images of soft agar colony formation assays for DKK1 knockdown experiments (top) and quantification of colony number (bottom). *p < 0.05, **p < 0.01. Data are mean ± SEM. (n = 3). C Representative images of tumor sphere formation assays for DKK1 knockdown experiments (top) and quantification of tumor sphere number (bottom). ***p < 0.001. Data are mean ± SEM. (n = 3). D Representative western blots of SW48-OR cells stably expressing either a scrambled shRNA or shRNAs specific for CKAP4 (left) and quantification (right). β-Actin served as a loading control. *p < 0.05, **p < 0.01, ***p < 0.001. Data are mean ± SEM. (n = 3). E Representative images of soft agar colony formation assays for CKAP4 knockdown experiments (top) and quantification of colony number (bottom). *p < 0.05. Data are mean ± SEM. (n = 3). F Representative images of tumor sphere formation assays for CKAP4 knockdown experiments (top) and quantification of tumor sphere number (bottom). *p < 0.05, **p < 0.01. Data are mean ± SEM. (n = 3).
