Fig. 5: The effect of Morusinol on polarization of macrophages in vitro and in vivo.
From: Targeting fucosyltransferase FUT8 as a prospective therapeutic approach for DLBCL
A THP-1 cells were exposed to PMA (100 ng/mL) for 24 h, then M1 and M2 polarization was treated with LPS (100 ng/mL) plus IFN-γ (20 ng/mL) and IL-4 (20 ng/mL) respectively, with or without Morusinol (6.508μm/L) for 24 h. Heatmap of differentially expressed genes between groups of macrophages. B Mfuzz clustering analysis of macrophages. C Quantitative real-time PCR was performed to assess the mRNA levels of M1-marker and M2-marker genes. D–F Expressions of specific M1 and M2 signature proteins were assessed using ELISA assay and flow cytometry. G There were 5 mice in each group. Tumor specimens photographed with a high-definition digital camera. Tumor volume was measured every 2 days. The weights of the nude mice were recorded every 2 days. H The tumor tissues from a different group were double stained with the macrophage marker F4/80 and the M1-marker CD86/M2-marker CD206. The percentage of CD86+ and CD206+ cells was calculated as a ratio of red cells to DAPI blue cells. All values are shown as the means SD based on triplicate measures. ***P < 0.001.
