Fig. 1: Mammary-specific deletion of Jagged1 in virgin mice resulted in reduced Notch activation in the luminal compartment, associated with expansion of MaSC and decreased luminal differentiation. | Oncogenesis

Fig. 1: Mammary-specific deletion of Jagged1 in virgin mice resulted in reduced Notch activation in the luminal compartment, associated with expansion of MaSC and decreased luminal differentiation.

From: Subtype-specific role for Jagged1 in promoting or inhibiting breast tumor formation

Fig. 1

A X-gal staining of mammary tissue sections from 6-week-old virgin Jag1β-Geo/+ mice showing TEB (upper) and mature duct (lower). B Representative flow cytometry analysis of lineage-depleted mammary cells isolated from virgin MMTV-Cre;TNR and Jag1loxP/loxP;MMTV-Cre;TNR mice at 7–10 weeks of age, with quantitation of GFP-positive cells in the CD24+CD49fhi (basal), CD24hiCD49flo (luminal), and CD24−CD49f− (stromal) compartments. MMTV-Cre mammary glands serve a negative control for GFP. C Flow cytometry analysis of lineage-depleted mammary cells from virgin MMTV-Cre and Jag1loxP/loxP;MMTV-Cre mice at 7–10 weeks of age and comparison of the CD24+CD49fhiCD61+Sca1− (MaSC) and CD24hiCD49floSca1+ (differentiated luminal cell) subpopulations between the two genotypes. D–F Immunostaining and Western blot analysis for ERα and PR in the mammary glands of MMTV-Cre and Jag1loxP/loxP;MMTV-Cre mice at 12 weeks of age. Scale bars: 50 μm. *p < 0.05; **p < 0.01 (Student’s t-test).

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