Fig. 5: Antioxidant defenses are increased in relapsed tumors and in tumors during treatment. | Oncogenesis

Fig. 5: Antioxidant defenses are increased in relapsed tumors and in tumors during treatment.

From: Adaptation of redox metabolism in drug-tolerant persister cells is a vulnerability to prevent relapse in pancreatic cancer

Fig. 5

A Intracellular levels of reduced glutathione (GSH), oxidized glutathione (GSSG), and total glutathione (GSH + GSSG) were measured with the GSH/GSSG-Glo™ Assay (Promega kit) in PDAC relapsed tumors, and the ratio GSH/GSSG was calculated. RT-qPCR analysis of antioxidant gene expression: Nrf2, HO-1, SLC7A11, and GPX4, in relapsed PDAC tumors at endpoint (B), under treatment (C) and start of relapse (D). E The mRNA levels of PGC-1α and TFAM was monitored by RT-qPCR in PDAC tumors under treatment and start of relapse, respectively. Each dot corresponds to the mean of triplicates for one mouse, and the bars show the mean ± SD. Data are representative of three independent qPCR experiments. Significance was determined by unpaired Student’s t test. *, **, *** and **** correspond to p < 0.05, 0.01, 0.001, and 0.0001, respectively; ns non-significant difference.

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