Fig. 1

The generation of agonist dose response curves from neonatal platelets. a Blood from neonatal donors from an arterial line, capillary or vein was incubated with ADP and platelet fibrinogen binding was measured (mean, ± SEM). b, c Platelets from 28 neonates were activated with ADP (b) or CRP-XL (c) and P-selectin exposure was measured as percentage of positive platelets or platelet mean fluorescence intensity (MFI) (mean, ± SEM). d–g Platelets from three healthy adult donors were activated with ADP (d, f) or CRP-XL (e, g) and percentage of fibrinogen binding (d, e) or MFI of exposed P-selectin (f, g) was measured (mean, ±SEM). Two lag delay periods were artificially introduced: (1) time between sample fixation and running the sample on the flow cytometer (1, 4, 8 and 16 h), and(2) the time between venipuncture and platelet stimulation extended to 16 h, after which they were immediately fixed and read on the flow cytometer. h Platelet count was plotted against the gestation of 28 neonates and the linear regression co-efficient (R²) was calculated