Abstract
Background
Chronic undernutrition leads to growth hormone resistance and poor growth in children, which has been shown to be modulated by microbiota. We studied whether Lactobacillus fermentum CECT5716 (Lf CECT5716), isolated from mother’s breast milk, could promote juvenile growth through the modulation of lipid absorption in a model of starvation.
Methods
Germ-free (GF) Drosophila melanogaster larvae were inoculated with Lf CECT5716 in conditions of undernutrition with and without infant formula. The impact of Lf CECT5716 on larval growth was assessed 7 days after egg laying (AED) by measuring the larval size and on maturation by measuring the emergence of pupae during 21 days AED. For lipid absorption test, Caco2/TC7 intestinal cells were incubated with Lf CECT5716 and challenged with mixed lipid micelles.
Results
The mono-associated larvae with Lf CECT5716 were significantly longer than GF larvae (3.7 vs 2.5 mm; p < 0.0001). The effect was maintained when Lf CECT5716 was added to the infant formula. The maturation time of larvae was accelerated by Lf CECT5716 (12 vs 13.2 days; p = 0.01). Lf CECT5716 did not have significant impact on lipid absorption in Caco2/TC7 cells.
Conclusions
Lf CECT5716 is a growth-promoting strain upon undernutrition in Drosophila, with a maintained effect when added to an infant formula but without effect on lipid absorption in vitro.
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Acknowledgements
P.P. received research support as a second-year master’s student from LACTALIS NUTRITION SANTE® France. P.P would like to thank Gwenael Jan from the French National Institute for Agriculture, Food and Environment (UMR STLO, Rennes, France) for his precious help in developing the cell co-culture model.
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P.P., N.P. and F.L. have contributed to the conception and design of the study. P.P., A.P., M.C.M., E.M., V.S., L.M., and C.L.-P have contributed to acquisition of data. All authors have contributed to the analysis and interpretation of data. P.P. drafted the article. All authors reviewed and revised it critically for intellectual content and gave their final approval of the version to be published. P.P. would like to thanks Gwenaël Jan for his precious advice in cell and bacteria co-culture.
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Poinsot, P., Penhoat, A., Mitchell, M. et al. Probiotic from human breast milk, Lactobacillus fermentum, promotes growth in animal model of chronic malnutrition. Pediatr Res 88, 374–381 (2020). https://doi.org/10.1038/s41390-020-0774-0
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DOI: https://doi.org/10.1038/s41390-020-0774-0
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