Abstract
Background
The survival of antibody isotypes specific to pertussis toxin (PT) and filamentous hemagglutinin (FHA) from mother’s own milk (MBM) and donor breast milk (DBM) during preterm infant digestion was investigated.
Methods
Feed, gastric, and stool samples were collected from 20 preterm mother–infant pairs at 8–9 days and 21–22 days postpartum. Samples were analyzed via ELISA for anti-FHA or anti-PT immunoglobulin A (IgA), IgM, and IgG.
Results
Anti-PT IgA, anti-FHA IgG, and anti-PT IgG were lower in MBM than DBM at 8–9 days postpartum, whereas anti-FHA IgM was higher in MBM than DBM. Anti-PT IgA, anti-PT IgG, and anti-FHA IgG in DBM decreased in gastric contents at both postpartum times but those antibodies in MBM were stable or increased during gastric digestion. Anti-FHA-specific IgA and IgM were higher in gastric contents from infants fed MBM than from infants fed DBM at 8–9 days. All pertussis antibodies were detected in infant stools at both postpartum times.
Conclusions
Pertussis-specific antibodies from MBM were stable during infant digestion, whereas anti-pertussis IgA and IgG from DBM decreased in gastric contents. The constant region and variable region of antibodies and maternal immunization appear to be the critical factors for their stability to proteolytic digestion and pasteurization.
Impact
-
Pertussis-specific antibodies from mother’s breast milk were stable during infant digestion, whereas anti-pertussis IgA and IgG from donor breast milk decreased in gastric contents.
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The constant region and variable region of pertussis-specific antibodies and the maternal immunization (previous infections and vaccinations) appear to be the critical factors for their stability to proteolytic digestion and pasteurization.
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Pertussis-specific antibodies from either mother’s breast milk or donor breast milk survived during preterm infant digestion and both types of milk will compensate for the lower IgG transplacental transfer in preterm infants compared with term infants.
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Acknowledgements
We thank the Northwest Mother’s Milk Bank for their support. This study was funded by the K99/R00 Pathway to Independence Career Award, Eunice Kennedy Shriver Institute of Child Health & Development of the National Institutes of Health (R00HD079561), and The Gerber Foundation.
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V.D.-M., D.C.D., and R.K.H. designed the clinical study. V.D.-M. conducted the experiments and the statistical analyses and wrote the paper. R.K.H., A.M.M., E.A.M., and R.L.M. provided milk and gastric samples and revised the paper. All authors revised the paper.
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Demers-Mathieu, V., Huston, R.K., Markell, A.M. et al. Impact of pertussis-specific IgA, IgM, and IgG antibodies in mother’s own breast milk and donor breast milk during preterm infant digestion. Pediatr Res 89, 1136–1143 (2021). https://doi.org/10.1038/s41390-020-1031-2
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DOI: https://doi.org/10.1038/s41390-020-1031-2
