Fig. 2: Necrostatin-1s ameliorated NEC in animal models.
From: Toll-like receptor 4-mediated necroptosis in the development of necrotizing enterocolitis
a The survival rate of neonatal mice in each group. n = 10 in either of the BF groups and n = 18 in either of the NEC groups. b Typical hematoxylin and eosin staining of the distal ileum in each group, magnification = ×100, bar = 100 μm. c The Nadler’s grade, n = 10 in each group. NEC + DMSO vs. NEC + Nec-1s, p = 0.001. d Real-time PCR showing the expressions of the inflammatory cytokines, n = 6 in each group. Statistical comparisons were done between the NEC + DMSO group and the NEC + nec-1s group. BF breastfeeding under a physiological status, NEC neonatal mice that underwent formula feeding and cold/asphyxia stress, DMSO 10% DMSO in PBS, the dissolvent of necrostatin-1s, nec-1s necrostatin-1s. NEC + DMSO vs. NEC + Nec-1s, #p > 0.05, *p < 0.05, and **p < 0.01.
