Fig. 1: HIF-1α expression in cord blood and adult mononuclear cells under anoxia.

Peripheral blood mononuclear cells (PBMC) and cord blood mononuclear cells (CBMC) were isolated and cultured under normoxia or under anoxia for 4 (a) or 48 (b) hours. Expression of HIF-1α was analyzed on protein level via western blot and on RNA level via PCR. a Representative western blot shows expression of HIF-1α and housekeeping gene GAPDH under anoxia in PBMC (left line) and CBMC (right line). Bar plot shows HIF-1α protein expression under anoxia in western blot assessed by densitometry in PBMC and CBMC. Bars represent pooled data from 14 independent experiments and standard deviation is depicted. ****p < 0.0001; Wilcoxon matched-pairs signed rank test. b Dot plot with bars shows HIF-1α mRNA expression under normoxia and anoxia in PBMC and CBMC relative to the housekeeping gene RPL37A assessed by qPCR. Bars represent pooled data from 6 independent experiments and mean is depicted. ns not significant; Wilcoxon matched-pairs signed rank test. c Representative western blot shows expression of PHD II and housekeeping gene GAPDH under normoxia and anoxia in PBMC (left two lines) and CBMC (right two lines). Dot plot with bars shows PHD II protein expression under normoxia and anoxia in western blot assessed by densitometry in PBMC and CBMC. Bars represent pooled data from 7 to 9 independent experiments and mean is depicted. ns not significant; Kruskal–Wallis test.