Abstract
Background
Special AT-rich sequence-binding protein 2 is essential for the development of cerebral cortex and key molecular node for the establishment of proper neural circuitry and function. Mutations in the SATB2 gene lead to SATB2-associated syndrome, which is characterized by abnormal development of skeleton and central nervous systems.
Methods
We generated Satb2 knockout mouse model through CRISPR-Cas9 technology and performed RNA-seq and ChIP-seq of embryonic cerebral cortex. We conducted RT-qPCR, western blot, immunofluorescence staining, luciferase reporter assay and behavioral analysis for experimental verification.
Results
We identified 1363 downstream effector genes of Satb2 and correlation analysis of Satb2-targeted genes and neurological disease genes showed that Satb2 contribute to cognitive and mental disorders from the early developmental stage. We found that Satb2 directly regulate the expression of Ntng1, Cdh13, Kitl, genes important for axon guidance, synaptic formation, neuron migration, and Satb2 directly activates the expression of Mef2c. We also showed that Satb2 heterozygous knockout mice showed impaired spatial learning and memory.
Conclusions
Taken together, our study supportsroles of Satb2 in the regulation of axonogenesis and synaptic formation at the early developmental stage and provides new insights into the complicated regulatory mechanism of Satb2 and new evidence to elucidate the pathogen of SATB2-associated syndrome.
Impact
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1363 downstream effector genes of Satb2 were classified into 5 clusters with different temporal expression patterns.
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We identified Plxnd1, Ntng1, Efnb2, Ephb1, Plxna2, Epha3, Plxna4, Unc5c, and Flrt2 as axon guidance molecules to regulate axonogenesis.
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168 targeted genes of Satb2 were found to regulate synaptic formation in the early development of the cerebral cortex.
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Transcription factor Mef2c is positively regulated by Satb2, and 28 Mef2c-targeted genes can be directly regulated by Satb2.
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In the Morris water maze test, Satb2+/− mice showed impaired spatial learning and memory, further strengthening that Satb2 can regulate synaptic functions.
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Data availability
RNAseq and ChIP-seq data that support the findings of this study have been deposited to GEO database with the accession number GSE201562.
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Acknowledgements
We are very grateful to Dr. Zilong Qiu and Dr. Man Xiong for their comments and suggestions during the study. We are also grateful to the animal co-facility center of Children’s Hospital of Fudan University and our genetic laboratory teams who contributed to this study.
Funding
This study is funded by the National Natural Science Foundation of China (81471483, 81974237).
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H.W. designed the study and revised the manuscript; Q.G. performed experiment, collected and interpreted the data, drafted the initial manuscript; Y.W. analyzed the RNA-seq and ChIP-seq data; Y.Q., X.C. and X.L. involved experiment, X.D. and H.C. involved data analysis, Q.W., Y.J., S.Y., J.Z., and S.S. involved animal experiment; B.W. and W.Z. revised the manuscript; and all authors read and approved the manuscript.
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Guo, Q., Wang, Y., Wang, Q. et al. In the developing cerebral cortex: axonogenesis, synapse formation, and synaptic plasticity are regulated by SATB2 target genes. Pediatr Res 93, 1519–1527 (2023). https://doi.org/10.1038/s41390-022-02260-z
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DOI: https://doi.org/10.1038/s41390-022-02260-z
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