Abstract
Background
To study the associations of Protein Tyrosine Phosphatase-N1 (PTPN1) polymorphisms with obesity-related phenotypes in European adolescents, and the influence of physical activity on these relationships.
Methods
Five polymorphisms of PTPN1 were genotyped in 1057 European adolescents (12–18 years old). We measured several phenotypes related to obesity, such as adiposity markers, and biochemical and clinical parameters. Physical activity was objectively measured by accelerometry.
Results
The T, A, T, T and G alleles of the rs6067472, rs10485614, rs2143511, rs6020608 and rs968701 polymorphisms, respectively, were associated with lower levels of obesity-related phenotypes (i.e., body mass index, body fat percentage, hip circumference, fat mass index, systolic blood pressure and leptin) in European adolescents. In addition, the TATTG haplotype was associated with lower body fat percentage and fat mass index compared to the AACCA haplotype. Finally, when physical activity levels were considered, alleles of the rs6067472, rs2143511, rs6020608 and rs968701 polymorphisms were only associated with lower adiposity in active adolescents.
Conclusions
PTPN1 polymorphisms were associated with adiposity in European adolescents. Specifically, alleles of these polymorphisms were associated with lower adiposity only in physically active adolescents. Therefore, meeting the recommendations of daily physical activity may reduce obesity risk by modulating the genetic predisposition to obesity.
Impact
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Using gene-phenotype and gene*environment analyses, we detected associations between polymorphisms of the Protein Tyrosine Phosphatase-N1 (PTPN1) gene and obesity-related phenotypes, suggesting a mechanism that can be modulated by physical activity.
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This study shows that genetic variability of PTPN1 is associated with adiposity, while physical activity seems to modulate the genetic predisposition.
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This brings insights about the mechanisms by which physical activity positively influences obesity.
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Data availability
In order to maintain a low risk of re-identification and compliance with the EU General Data Protection Regulation, the clinical and genetic raw data used in this study cannot be public. Interested parties can request the Risk Analysis Assessment approved by the funding agency that includes full explanations about the security measures and their justification.
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Acknowledgements
We wish to thank Rosa Maria Torres, Petra Pickert, and Anke Berchtold for their contribution to laboratory work.
Funding
The HELENA Study was supported by contract FOOD-CT-2005-007034 from the European Community Sixth RTD Framework Programme. The present study was also supported by a Marie S. Curie Global Fellowship within the European Union research and innovation framework programme (2014–2020; ClimAHealth; https://doi.org/10.3030/101030971), by a grant from the Spanish Ministry of Science and Innovation (AGL2007-29784-E), by Redes Temáticas de Investigación Cooperativa RETIC grant (Red SAMID RD16/0022), by the University of Granada Plan Propio de Investigación (Excellence actions: Unit of Excellence on Exercise and Health [UCEES]), by the Junta de Andalucía, Consejería de Economía, Conocimiento, Empresas y Universidad (refs. P18–624 RT-4455, SOMM17/6107/UGR), and by grants from the Public University of Navarra, “Ayudas a Grupos de Investigación (2019)”. The content of this article reflects only the authors’ views, and the European Community is not liable for any use that may be made of the information contained therein.
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I.L., M.G.-G., M.S.-C., L.A.M., A.G.Z., K.W., A.M., D.E., D.F.S.-T. and J.R.R. designed the study. D.F.S.-T. performed all analyses. D.F.S.-T. and J.R.R. wrote the initial draft and all co-authors significantly contributed to the final version.
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Salazar-Tortosa, D.F., Labayen, I., González-Gross, M. et al. Association between PTPN1 polymorphisms and obesity-related phenotypes in European adolescents: influence of physical activity. Pediatr Res 93, 2036–2044 (2023). https://doi.org/10.1038/s41390-022-02377-1
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DOI: https://doi.org/10.1038/s41390-022-02377-1
