Fig. 3: ROS production of cord blood monocytes after stimulation with Lactobacillus rhamnosus. | Pediatric Research

Fig. 3: ROS production of cord blood monocytes after stimulation with Lactobacillus rhamnosus.

From: Different probiotic strains alter human cord blood monocyte responses

Fig. 3

CBMC were isolated, cultured overnight and stimulated with Lactobacillus rhamnosus (LR) in a MOI of 1:0.1 and 1:1 for 1 h. Production of ROS was assessed after staining with dihydrorhodamine (DHR) by flow cytometry. a Representative histogram shows expression of rhodamine in unstimulated monocytes (white) and monocytes after stimulation with LR (grey) in a MOI of 1:1. b Scatter plot with bars show the mean fluorescent intensity (MFI) for expression of Rhodamine in cord blood monocytes without stimulation (white bar) and after stimulation of LR in different MOIs (grey bars). n = 8, **p < 0.01, paired t test.

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