Fig. 4: Expression of cytokines by cord blood monocytes after stimulation with Lactobacillus rhamnosus and LPS.
From: Different probiotic strains alter human cord blood monocyte responses
CBMC were isolated, cultured overnight and stimulated with Lactobacillus rhamnosus (LR) in a MOI of 1:0.1 and 1:1 for one hour. Afterwards brefeldin and LPS were added and cells were cultured for another four hours. Cells stimulated with LPS alone served as control. Expression of IL-1β, IL-8, TNF-α and TGF-β was determined after intracellular staining by flow cytometry. a, c, e, g Scatter plots with bars show percentages of cord blood monocytes expressing IL1β (a), IL-8 (c), TNF-α (e) and TGF-β (g) after stimulation with LPS alone (black bars) and after stimulation of LR in different MOIs (grey bars). b, d, f, h Scatter plots with bars show the mean fluorescent intensity (MFI) for expression of IL-1β (b), IL-8 (d), TNF-α (f) and TGF-β (h) in cord blood monocytes after stimulation with LPS alone (black bars) and after stimulation of LR in different MOIs (grey bars). n = 10, *p < 0.05, **p < 0.01, ns not significant; Wilcoxon matched-pairs signed-rank test (a, c, e, g) and paired t test (b, d, f, h).
