Fig. 1: Summary of the experimental protocol.
From: Early assessment of injury with optical markers in a piglet model of neonatal encephalopathy
Male newborn piglets <60 h of age were randomised into control (no HI), moderate HI or severe HI groups. HI groups achieved severity levels based on the duration of HI combined with key physiological and biochemical thresholds. HI groups also underwent 15 min of baseline neuro/optical and systemic monitoring prior to the start of HI insult and all groups underwent 6 h of continuous monitoring with bNIRS/DCS, EEG and systemic monitoring whilst receiving intensive care. At 6 h, all piglets had 1H MRS imaging to attain BGT and WM Lac/NAA outcome before sacrifice and tissue collection for TUNEL histochemical staining. Wavelet analysis was then carried out on all signals over a 60 min data period commencing at 1 h following the start of HI or at the same time in controls.
