Fig. 5: H&E and IF staining of colon tissue in the control group and narrow segment tissue in the treatment group. | Pediatric Research

Fig. 5: H&E and IF staining of colon tissue in the control group and narrow segment tissue in the treatment group.

From: Establishment and identification of an animal model of Hirschsprung disease in suckling mice

Fig. 5: H&E and IF staining of colon tissue in the control group and narrow segment tissue in the treatment group.

H&E staining showed that the ganglion of the intermuscular plexus was ablated, but the other tissues were intact. In addition, thickening of muscle layer tissue and a small amount of inflammatory cell infiltration were found (objective lens: ×10). IF staining showed that the expression of ganglion-specific marker PGP9.5 gradually decreased to disappearance (objective lens: ×10).

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