Fig. 5: Changes in genes and proteins after overexpression of miR-27a-3p in HEK-293T cells (n = 3).

Data are presented as mean ± SEM (n = 3), *p < 0.05, **p < 0.01, and ***p < 0.001 compared with the control group. a The mRNA expression levels of GOLM1, LIMK1, and CBX1 in HEK-293T cells after overexpression of miR-27a-3p, with β-actin used as an internal reference standard. b Quantitative analysis of GOLM1, LIMK1, and p-LIMK1 protein levels in HEK293 cells (normalized to GAPDH). Representative WB results are shown. c The expression levels of miR-27a-3p in mouse plasma were determined 1 h after epilepsy was terminated, with the external standard used as a reference control; the expression level of miR-27a-3p in mouse brain 6 h after epilepsy was terminated, with U6 used as a reference. The expression of miR-27a-3p in plasma and whole brain in the epilepsy group was upregulated. d RNA expression levels of GOLM1 and LIMK1 in the whole brain of mice 6 h after epilepsy was terminated (GAPDH was used as an internal reference for GOLM1 and LIMK1). e Representative immunofluorescence staining images of GOLM1, LIMK1, and P-LIMK1 (green) in mouse hippocampus 6 h after epilepsy termination. Staining levels of GOLM1, LIMK1, and P-LIMK1 were reduced in the epilepsy group (brain scale bars, 500 μm; hippocampal scale bars, 100 μm). f Quantification data of mean fluorescence intensity of mouse hippocampal CA1, CA3, DG region of GOLM1 (n = 3), LIMK1 (n = 3), and P-LIMK1 (n = 3). Data are presented as mean ± SEM; *p < 0.05 and **p < 0.01 vs. the control group.