Fig. 2: Classification of protein levels changing after birth in preterm infants.

a Graphical visualisation of the approach to classifying proteins that show changes over time based on 1) similarity score (top left) calculated as the squared Pearson correlation to theoretical changes over time and 2) effect size (bottom left) calculated as the area of the protein level changes over time normalised to the protein levels at day 0 based on imputed data. b The label free quantification (LFQ)-intensity levels for immunoglobulin heavy gamma 1 (IGHG1, purple) and haemoglobin subunit gamma-2 (HBG2, green) per time point in infants with each sample shown using dots and the distribution with Tukey box-and-whisker plot. On the right the similarity score and effect size is depicted. c Similarity score plotted against the effect size for each protein represented as a dot. Red dots indicate haemoglobins and blue dots immunoglobulins, all other proteins are represented in grey dots. The squared in the plot show the threshold set for the classification of proteins for which the levels decrease (orange) or increase (green) with time after birth. d Tukey box-and-whisker plot representing the distribution of LFQ-intensity levels of coagulation factor XIIIa (F13A1), sulfhydryl oxidase 1 (QSOX1), bifunctional purine biosynthesis protein (ATIC), porphobilinogen deaminase (HMBS), complement component 9 (C9) and apolipoprotein A-IV (APOA4) per timepoints. Proteins panel backgrounds represent their classification. e Enrichment network visualization based on proteins classified as decreasing (orange) or increasing (green) with time with manual annotation of highlighted enrichment terms. Nodes represented each term within by pie charts indicating their associations with each classification.