Fig. 3: Effects of HM EVs on Caco-2 intestinal cells. | Pediatric Research

Fig. 3: Effects of HM EVs on Caco-2 intestinal cells.

From: Human milk extracellular vesicles modulate inflammation and cell survival in intestinal and immune cells

Fig. 3

a Cell viability following treatment with term HM EVs, or media only control for 6 or 24 h. Viability measured with XTT assay - absorbance (450 nm), n = 4–9, *p < 0.04, two-way ANOVA with Tukey’s test, two replicate experiments. b EGF levels in cell media following inflammatory activation with heat-killed bacteria (HKB, 109 HKU) on cells treated for 22 h with term or preterm HM EVs (20 μg/ml), or media only control. Regardless of inflammatory activation with HKB, both term and preterm HM EVs significantly increased levels of EGF. Term HM EVs resulted in significantly higher levels when compared to preterm, n = 3. c CD14 surface expression normalized to HKB treatment, median fluorescence intensity (MFI) of BV421, n = 3, ns: p = 0.09. Plot is representative of two replicate experiments. d Soluble CD14 levels in media were significantly increased by term and preterm HM EVs, regardless of inflammatory activation with HKB, n = 3-4. ***p < 0.001, **p < 0.01, *p < 0.04, ns=non-significant, one-way ANOVA.

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