Fig. 5: Protein expression for genes that changed in the fetal lung in response to antenatal Vitamin C administration. | Pediatric Research

Fig. 5: Protein expression for genes that changed in the fetal lung in response to antenatal Vitamin C administration.

From: Antenatal Vitamin C differentially affects lung development in normally grown and growth restricted sheep

Fig. 5

Data presented as normalized protein expression in arbitrary units (AU) for SP-B (a; 8 kDa band), ENAC-ß (b; 87 kDa band), Na-K-ATPase-α1 (c; 110 kDa band), Na-K-ATPase-ß1 (d; 50 kDa band), 11ßHSD-2 (e; 44 kDa band), GLUT-1 (f; 46 kDa band). Western blot images represent target protein (upper panel) and reference protein (lower panel) for fetuses in normoxic + saline (NS), normoxic + Vitamin C (NVC), hypoxic + saline (HS) and hypoxic + Vitamin C (HVC) fetuses. ⊗ = Outliers (all data were evaluated for outliers ± 2 standard deviations from mean of each treatment group) not included in analysis. Beta actin (ß-actin; h, i, k, l; 42 kDa band), Cytochrome oxidase IV (COXIV; j; 17 kDa band), and beta-tubulin (ß-tubulin; g; 55 kDa band) are obtained from the same gel. Positive or negative mean values indicate induction or repression of gene expression, respectively. P < 0.05 was considered statistically significant.

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