Fig. 4

Repression motifs are required for the regulation of E-cadherin expression by Tbx3. a A low level of E-cadherin expression was found in 53 paired primary HCC tissues compared with adjacent non-tumor tissues by RT-qPCR. b Negative correlations were observed between Tbx3 and E-cadherin expression in clinical HCC samples subjected to RT-qPCR; statistical analysis was performed using SPSS software. c, d According to WB (C) and RT-qPCR (D) assays, Tbx3 repressed E-cadherin expression whereas the Tbx3 mutant had no obvious effect on E-cadherin expression in HepG2 cells