Fig. 6 | Signal Transduction and Targeted Therapy

Fig. 6

From: Novel HDAC5-interacting motifs of Tbx3 are essential for the suppression of E-cadherin expression and for the promotion of metastasis in hepatocellular carcinoma

Fig. 6The alternative text for this image may have been generated using AI.

Tbx3 interacts with HDAC5 through the 585LFSYPYT591 and 604HRH606 motifs. a, c An antibody against HA-HDAC5 (a) or the DNA-binding domain of Gal4 (c) was used to precipitate HDAC5- or RD-binding proteins; the RDs (a) or HDAC5 (c) were detected by western blot using a specific antibody against the Gal4- or HA-tag (upper panel). The input HA-HDAC5 or RD was detected by a specific antibody against the HA- or Gal4-tag; b, d The relative protein amounts in A (RD vs HDAC5) and in C (HDAC5 vs RD). The image was scanned, and the density of each band was calculated using Quantity One software. The mean values of three independent experiments are presented with standard deviations; e A summary of HDAC5 binding activities and repression activities of the wild type and mutated RD domains of Tbx3; f 293 T cells were transfected with Myc-Tbx3 (WT or mutant form) and HA-HDAC5 plasmids. Cell lysate was subjected to IP with the indicated antibody against Myc or HA tag; g A proposed working model for Tbx3 and HDAC5 in HCC

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