Fig. 2

Mdig affects cell motility and invasion of noncancerous breast and breast cancer cells. Transwell migration (a) and Matrigel invasion (b) assay of indicated cell lines following transfection with Ctrl siRNA, mdig siR2 or mdig siR5 24 h post transfection. Scale bar = 200 μM. Quantification of number of migrated cells and invaded cells depicted by a bar graph below each of the figure panels. Ten randomly selected fields were counted for migrated and invasive cells (n = 10). * p < 0.05; **p < 0.01. c. Western blotting shows a decrease in mdig protein for indicated cell lines 24 h after knockdown of mdig by siRNAs