Fig. 6

BAFF-Trap regulates the activation and polarization of T cells, suppresses the activation of DCs. a Percent of T-cell subsets in axillary and popliteal lymph nodes. Bars represent mean ± S.D. (n = 5). On day 42 after the indicated treatments, mice were killed to obtain lymph nodes. Then the cells in lymph nodes were incubated with corresponding antibodies to detect the subsets of T cells. Bars represent mean ± S.D. (n = 5). b Percentage of Th1 and Th17 cells in total joint cells. On day 42 after treatment, the joints were obtained and digested by collagenase type IV. Then Th1 (CD4⁺IFN-γ⁺) and Th17 (CD4⁺IL-17⁺) cells from joints were analysed by FACS. Bars represent mean ± S.D. (n = 5). c Percentage of Treg cells and DCs in total joint cells. The joint cells were obtained as mentioned in b. CD4⁺CD25⁺Foxp3⁺ meant Treg cells, CD11b⁺CD11c⁺ meant activated DCs. Bars represent mean ± S.D. (n = 5). d Representative FACS analysis of DCs. **P < 0.01 versus PBS; ^##P < 0.01 versus normal