Fig. 3

oxLDL induced IDO1 expression via the PI3K/Akt/NF-κB pathway in THP-M. a oxLDL induced the production of inflammatory factors. The mRNA levels of IL-10, MCP-1, IL-1β and IL-8 were measured by real-time PCR. b THP-M were challenged with 25 mg/L oxLDL for 12, 24, and 48 h, and IDO1 expression was detected by western blot analysis. c THP-M were treated with oxLDL for 24 h at 0, 25, 50 and 100 mg/L, and IDO1 expression was detected by western blot analysis. d The expression of PI3K/Akt/NF-κB pathway-related proteins in THP-M was detected by western blot analysis. THP-M were treated with oxLDL (25 mg/L) for 24 h. e The expression levels of pAkt, pp65, IDO1 and β-actin were detected by western blots. THP-M were pretreated with the Akt inhibitor LY294002 (5 μM) for 24 h and then treated with oxLDL (25 mg/L) for 24 h. Data were based on at least three independent experiments. *p < 0.05. f The expression levels of pp65, IDO1 and β-actin were detected by western blots. THP-M were pretreated with the NF-κB inhibitor CAPE (50 μM) for 24 h and then treated with oxLDL (25 mg/L) for 24 h