Fig. 4

Before and after oxLDL treatment, the promoting effects of IFN-γ on IDO1 expression and the degree of foaming of THP-M. a Induction of IDO1 by different treatments was detected by immunofluorescence (×200). b The degree of foaming was detected by Oil red O staining (×400). c Quantitative analysis of Oil red O intensity (Oil red O staining area/cell area and Oil red O staining particles per cell). The designation of different treatments, such as oxLDL, IFN-γ, IFN-γ+ oxLDL and oxLDL+IFN-γ, was described in the Materials and Methods. Data were based on at least three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001