Fig. 2

SQSTM1 knockdown sensitized NSCLC to gefitinib and AZD9291. Gefitinib (a) or AZD9291 (b) was administered to A549, HCC827, and H460 cells for 48 h after transfection with control siRNA (siNC) or SQSTM1 siRNA (siSQSTM1). Cell viability was measured by MTS assay. c A549, HCC827, and H460 cells were treated with gefitinib after 48 h of siSQSTM1 transfection. Western blotting was applied to measure c-PARP1 (cleaved PARP1) levels. d A549, HCC827, and H460 cells were treated with gefitinib after 48 h of siSQSTM1 transfection. The annexin V-PI staining assay was performed after the indicated treatment, and the relative percentage of apoptotic cells is summarized. e A549, HCC827, and H460 cells were treated with AZD9291 after 48 h of siSQSTM1 transfection. Western blotting was applied to measure c-PARP1 levels. f A549, HCC827, and H460 cells were treated with AZD9291 after 48 h of siSQSTM1 transfection. The annexin V-PI staining assay was performed after the indicated treatment, and the relative percentage of apoptotic cells is summarized. All statistical data are presented as the mean ± SD. Student’s t test was used for the statistical analysis, and a p value < 0.05 was considered statistically significant