Fig. 5 | Signal Transduction and Targeted Therapy

Fig. 5

From: The cross-talk between methylation and phosphorylation in lymphoid-specific helicase drives cancer stem-like properties

Fig. 5

ERK2-mediated LSH phosphorylation crosstalk with methylation of LSH by PRMT5. a Methyl R antibody immunoprecipitated proteins were applied to IB analysis using an anti-LSH antibody to detect LSH methylation in 293T cells transfected with LSH mutants including R309A as well as S503A, or LSH WT, which were treated without or with λPP. b Anti-LSH antibody immunoprecipitated proteins obtained from 293T cells transiently transfected with plasmids expressing LSH WT, R309A or S503A were subjected to IB assay with the generated LSH R309me1 antibody. c Anti-LSH antibody immunoprecipitated proteins obtained from PC9 cells stably expressing Vector, LSH WT, R309A or S503A were subjected to IB assay with the generated anti-LSH-pS503 antibody. d IP followed by IB assay in PC9 cell lines stably expressing LSH after treatment with the MAPK1/2 inhibitor LY3214996 for the indicated time. e Immunoprecipitated proteins derived from 293T cells transfected with LSH WT or LSH S503D were used for IB analysis with the anti-LSH-pS503 antibody and anti-Methyl R antibody. f IP followed by IB assay of interaction between PRMT5 and LSH WT or LSH mutants in PC9 cells stably expressing Vector, LSH WT, R309A or S503A

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