Fig. 1

TCP1 regulates Wnt7b/β-catenin pathway through P53 to influence the proliferation and migration of hepatocellular carcinoma cells. a Kaplan–Meier analysis of overall survival and disease-free survival of liver cancer patients. b Cell growth was measured using the CCK-8. c Transwell migration assay. d Cells were stained with PI for cell cycle analysis. e The tumor growth curves and the tumor weight of NC and sh-TCP1 mice. f The numbers of lung metastasis. g The differential gene expression profiles. h The significant signaling pathways of the differentially expressed genes. i Western blot analysis to detect the protein expression of the Wnt pathway. j Multiple luciferase report plasmids of the WNT7B promoter. k, l Potential P53 binding sites in the WNT7B promoter core region. m ChIP-qPCR. n CoIP analyze the interaction between TCP1 and P53. o TCP1 interacts with P53 to regulate Wnt7b/β-catenin pathway. Data represent the mean ± SEM. *P < 0.05, **P < 0.01, and ***P < 0.001; ns no significance