Fig. 4
From: COVID-19-activated SREBP2 disturbs cholesterol biosynthesis and leads to cytokine storm
SREBP-2 activation is critical for the vascular inflammatory responses via cholesterol release and cytokine expression. a Western blot analysis of N-term and C-term of SREBP-2 in whole cell lysate (WCL) and supernatant (Sup.) after the LPS stimulation (1 μg/ml). b Time-dependent NF-κB activation after the LPS stimulation (1 μg/ml) (**p < 0.01). c Time-dependent secretion of SREBP-2 C-term in WCL and Sup. after the LPS stimulation (1 μg/ml) (**p < 0.01). d Filipin staining after the LPS stimulation (Scale bar: 200 μm). e Western blot analysis of ATP-binding cassette transporter (ABCA1) in human umbilical vein endothelial cell (HUVEC) after the LPS stimulation (1 μg/ml). f Effect of SREBP-2 knockdown in the suppression of cytokine production. g Transendothelial permeability of HUVEC after the inhibition or overexpression (O/E) of relevant signaling (*p < 0.05, **p < 0.01)
