Fig. 1: MiR-126 is essential for liver regeneration through inhibiting the Cdkn2aip-p53 pathway.
From: Hepatic microRNA-126 deficiency restrains liver regeneration through p53 pathway in mice
a Relative expression of miR-126 in the perfusd hepatoctes before and after PH at different time points. Quantifications were normalized to U6. (0, 24h, 36h, 48h, and 72h). b Representative IHC staining of BrdU and Ki67 positive cells in Control and miR-126 LKO livers at 36h, 48h, and 72h following PH. Scale bar: 50 µm. c, d The quantification figures of the IHC staining of BrdU and Ki67 in control and miR-126 LKO livers at different time points (36h, 48h, and 72h) after PH. Five sections per mouse were examined, five mice per group. e Representative western blotting results of PCNA, p-p53, p21 in livers at different time points (36h, 48h) after PH. f, g The quantification figure of the western blotting analysis of PCNA, p-p53, p21 expression of livers in control and miR-126 LKO group at different time points (36 h, 48 h) after PH. Quantifications were normalized to β-actin (n ≥ 5 per group). h Representative and quantification figures of western blotting results of PCNA expression in PFT-α treated control and miR-126 LKO livers at 48 h, 72 h following PH. Quantifications were normalized to β-actin (n ≥ 4 per group). i, j Representative quantification figures of IHC staining of BrdU and Ki67 positive cells in PFT-α treated control and miR-126 LKO livers at 48h and 72h following PH. Scale bar: 50 µm. Five sections per mouse were examined, five mice per group. k Representative and quantification figures of western blotting results of Cdkn2aip in livers at different time points (36h, 48h) after PH. Quantifications were normalized to β-actin (n ≥ 5 per group). l RT-PCR analysis of cdkn2aip expression in control and miR-126 LKO mice 36h after PH. Quantifications were normalized to β-actin (n ≥ 5). Data are presented as mean±SEM. *P < 0.05; **P < 0.01; ***P < 0.001, Wilcoxon test in (k), student’s test in (a, c, d, f, g, h, j, l). PH partial hepatectomy, h hour, PCNAproliferating cell nuclear antigen, LKO liver specific knockout, N.S. no significant difference
