Fig. 5
Inhibition of CBS reduced endogenous H2S generation. a K562 cells were treated with AOAA (0.0, 0.2, 0.4, 0.8, 1.6, and 3.2 mM) for 48 h. Endogenous levels of H2S were detected by fluorescence assays (ex = 365 nm, em = 450 nm). b K562 cells were treated with different concentrations of AOAA for 48 h with or without NaHS (30 μM), caspase 3/7 activity was tested by using chemiluminescence method (n = 3). c The K562 cells were transfected with CBS-shRNA lentiviral vector (shCBS) or the control group (shNT) and the level of H2S was analyzed (n = 5). shNT and shCBS cells were treated with or not 1 mM AOAA for 48 h, cell viability (n = 6) and caspase 3/7 activity (n = 3) were detected by MTT (d) or chemiluminescence method (e), respectively. The results are expressed as mean ± SEM. Two-tailed Student’s t test was used to analyze the difference between 2 groups, and multiple comparisons were analyzed by ANOVA followed by Tukey’s multiple comparisons test
