Fig. 5
From: Pleiotropic tumor suppressor functions of WWOX antagonize metastasis
WWOX physically interacts with DVL2 and functionally results in reduced Wnt pathway activation. a WWOX-interacting proteins in MDA-MB435S cells. b Immunoblot showing the co-IP of WWOX and DVL2 from HEK293T cells using Flag tagged DVL2 and Myc-tagged WWOX; mouse IgG was used as a negative control. Control- without Wnt ligands, Wnt- with Wnt ligands. Heavy chain IgG is indicated for each lane to demonstrate equal amounts of precipitate. c qRT-PCR of AXIN2 in MDA-MB231 cells treated with or without Wnt ligands; UBC was used as an endogenous control. The number of times an experiment was repeated (N) is stated in the legend. d Immunoblot showing Fibronectin and WWOX in SUM149 cells; EV- empty vector, WWOX- wild-type WWOX, and WFPA- mutated WWOX. e qRT-PCR of AXIN2 in SUM149 cells treated with or without Wnt ligands; UBC was used as an endogenous control. GAPDH was used as an endogenous control in (a) and (c). **P-value < 0.01. Statistical analyses (including error bars and p values) for (c) and (e) represent three independent experiments
