Fig. 4

SARS-CoV2 specific CD4⁺ and CD8⁺ T cell responses in COVID-19 convalescent patients. PBMCs of healthy control (n=8) and recovered COVID-19 patients (n=64) were stimulated with SARS-CoV-2 dominant antigen (S1, S2 and nucleoprotein, N) cocktails for 44h, Golgi-Plug containing Golgi-stop and DNase were added into cell culture for another 4h. Samples of a, c, e, and g were from Healthy (#3), Asymptomatic (#4), Mild (#8), Moderate (#13), severe (#6). a FACS plot examples of IFNγ⁺CD4⁺ T cells in total live CD4⁺ T cells, gated on total live CD4⁺ T cells. b Bar graph shows the frequency of IFNγ⁺CD4⁺ T cells in total CD4⁺ T cells after stimulation, summarized from (a). c FACS plot examples of GZMB⁺Perforin⁺CD4⁺ T cells in total IFNγ⁺CD4⁺ T cells, gated on total live IFNγ⁺CD4⁺ T cells. d Frequency of GZMB⁺Perforin⁺CD4⁺ T cells in total IFNγ⁺CD4⁺ T cells, summarized from (c). e FACS plot examples of IFNγ⁺CD8⁺ T cells in total live CD8⁺ T cells, gated on total live CD8⁺ T cells. f Bar graph shows the frequency of IFNγ⁺CD8⁺ T cells in total CD8⁺ T cells after stimulation, summarized from (e). g FACS plot examples of GZMB⁺Perforin⁺CD8⁺ T cells in total IFNγ⁺CD8⁺ T cells, gated on total live IFNγ⁺CD8⁺ T cells. h Frequency of GZMB⁺Perforin⁺CD8⁺ T cells in total IFNγ⁺CD8⁺ T cells, summarized from (g). Bars represent the mean±SEM. P values were calculated based on Bonferroni of one-way ANOVA analysis. ***p<0.0001, **p<0.001, and *p<0.05