Fig. 2
From: Targeting circular RNAs as a therapeutic approach: current strategies and challenges
Strategies used to study circular RNA (circRNA). A CRISPR/Cas9-mediated circRNA knockout via removal of intronic complementary sequence flanking circularized exon involved in circRNA biogenesis. This system has also been used to target the entire gene locus and a transcription factor to knockout and knockdown circRNA, respectively (not shown). B Conditional circRNA knockdown mediated by a cre-dependent short hairpin RNA (shRNA), which is subsequently processed into short interfering RNA (siRNA) to induce circRNA cleavage. C CRISPR/Cas13-mediated circRNA knockdown directly targets the back-splice junction of circRNAs to induce circRNA cleavage. D CircRNA expression plasmid leads to circRNA overexpression. E siRNA/shRNA targeting the back-splice junction of circRNAs induces circRNA cleavage
