Fig. 2 | Signal Transduction and Targeted Therapy

Fig. 2

From: Bitter receptor TAS2R138 facilitates lipid droplet degradation in neutrophils during Pseudomonas aeruginosa infection

Fig. 2

TAS2R138 was involved in LDs reduction. WT or Tas2r138 siRNA-transfected AMs and neutrophils were infected with PAO1 (MOI: 5:1) for 2 h. a, b The LDs (stained by Nile red) expression was detected by immunofluorescence imaging in neutrophils (a) (arrows showed the typical LDs) and AMs (b). The mean fluorescence intensity (MFI) form 6–10 images were counted on the right by Image J. c The LDs were isolated from WT or infected and Tas2r138 siRNA-transfected neutrophils and the TAS2R138 on the LDs was detected by western blotting. e, f AMs and neutrophils were infected with PAO1 (MOI: 5:1) for 30 min or 4 h. The phagocytosis and clearance of PAO1 were counted by CFU. WT or Tas2r138 siRNA-transfected neutrophils were infected with PAO1 (MOI: 5:1) or AHL-12 (100 µM) stimulated for 2 h. The dead cells were stained by propidium iodide and counted by flow cytometry. The results were expressed as the mean ± SD and the significant difference level between two groups was defined by one-way ANOVA with Tukey post hoc tests, *p < 0.05

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