Fig. 3
Activating the PI3K-AKT pathway enhanced the megakaryopoiesis-supporting ability of macrophages from PT patients. a Schematic diagram of the study design on RNA-seq of the cultivated BM MФs from PT and GGF patients. b Heatmaps showed M1 and M2 scores (calculated by CIBERSORT algorithm) in PT MФs and GGF MФs (scaled by row). Box plot depicting c CIBERSORT score of M1 MФs, d CIBERSORT score of M2 MФs and e CIBERSORT score of M1/M2 in PT MФs and GGF MФs. f GO enrichment analysis showed the top ten terms enriched by the upregulated genes of GGF MФs and PT MФs. The size of each circle indicates the ratio of DEGs counts and the gene counts of the term. g KEGG enrichment analysis showed the top five pathways enriched by the upregulated genes of GGF MФs and PT MФs. h Heatmap showed expression of AKT1 gene in GGF MФs and PT MФs. i Representative western blots of p-AKT, AKT, and GAPDH expression in the cultivated BM MФs from PT patients and GGF patients. j Schematic diagram of the study design on RNA-seq of BM-M1 and BM-M2 from HD. k KEGG enrichment analysis showed the top five pathways enriched by the upregulated genes of M1 MФs and M2 MФs. The size of each circle indicates the scaled ratio of DEGs counts and the gene counts of the pathway. MK production and maturation, CFU-MK plating efficiencies, and platelet release were analyzed after coculture with BM MФs that were cultivated from PT patients and GGF patients and were subjected to various treatments. The l MK count, m MK ploidy distribution, n representative CFU-MK images (scale bars represent 50 μm), o CFU-MK count, and p platelet count were analyzed after 12 days of coculture. Data are presented as the means ± SEM (*P ≤ 0.05, ** P ≤ 0.01)
