Fig. 4: Transplanted MSCs undergo apoptosis within few hours and release phospholipid in vivo. | Signal Transduction and Targeted Therapy

Fig. 4: Transplanted MSCs undergo apoptosis within few hours and release phospholipid in vivo.

From: Spontaneous apoptosis of cells in therapeutic stem cell preparation exert immunomodulatory effects through release of phosphatidylserine

Fig. 4: Transplanted MSCs undergo apoptosis within few hours and release phospholipid in vivo.

a Here, 1 × 106 GFP-MSCs were injected into mice by i.v. and the lung and liver tissues were taken for frozen section and stained for C-Cas 3 (red) and DAPI (blue) at 0.5, 2, and 4 h. Scale bar represents 50 μm (upper row) or 10 μm (lower panel). b Quantification of living MSC number in the lung and liver after GFP-MSCs injection. **p < 0.01, ***p < 0.001, compared with the 0.5 h group. c Mice were injected with 1 × 106 MSCs (containing 5 × 104 DMSCs), or 5 × 104 DMSCs through tail vein. Plasma lipid was isolated at 0.5 or 4 h, and the lipid levels were detected by Nano high-resolution liquid mass analyzer. PCA plot on all samples using the normalized lipid levels of total lipids. d, e Fold change of total PS (d), PS (18:0/16:1)-H, PS (18:0/18:1)-H, PS (18:0/22:6)-H, PS (18:0/22:5)-H, PS (37:2)-H, and PS (39:1)-H (e) abundance after injection of MSCs and DMSCs at 0.5 and 4 h. Data are represented relative to the Control group. *p < 0.05, **p < 0.01, ***p < 0.001, compared with the Control group. c–e Every dot represents one individual animal. Data are represented as mean ± SEM. n = 4 in each group in b–e. ANOVA with Dunnett’s multiple comparison test was performed. C-Cas 3, cleaved Caspase 3

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