Fig. 1 | Signal Transduction and Targeted Therapy

Fig. 1

From: A novel inactivated whole-cell Pseudomonas aeruginosa vaccine that acts through the cGAS-STING pathway

Fig. 1

Morphology and biochemical characterization of XPa. a Transmission electron microscopy of PAO1, XPa (X-ray-treated PAO1), and formaldehyde-treated PAO1. Determination of nucleic acid (b) and 8-OHG (c) concentrations in the supernatant. d PI-stained PAO1-GFP after X-ray irradiation (XPa) and PAO1, and DNase I-treated XPa. e Nucleic acid size distribution in PAO1 and XPa (980 Gy) supernatants and the PAO1 genome were analyzed via agarose gel electrophoresis in the presence of a 2000-bp DNA ladder as a marker. f Molecular weight distribution curves according to e. g The supernatants of the PAO1 and XPa groups were separated on a 4–20% polyacrylamide TBE gel (Invitrogen, EC62255BOX), and run in 1× TBE buffer for 30 min. The gel was stained with SYBR Gold Nucleic Acid Gel Stain and detected using an enhanced chemiluminescence system (iBright CL1000, Thermo Fisher). Data were analyzed using an unpaired t-test. ***P < 0.001

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