Fig. 4
From: XBP1 regulates the protumoral function of tumor-associated macrophages in human colorectal cancer
Cytokines production induced by XBP1 activation in TAMs. a Representative cytokine arrays for sgCon miTAMs and paired sgXbp1 miTAMs supernatants. b Relative mRNA levels of cytokines in BMDMs, sgCon miTAMs, and paired sgXbp1 miTAMs validated by RT-qPCR. c Expression of cytokines in human TAMs. Human macrophages from THP-1 cells were induced to TAMs via incubation in condition medium of HCT116 cells, and relative mRNA levels of cytokines in THP-1, sgCon or sgXBP1 TAMs were validated by RT-qPCR. d ChIP-qPCR experiments measuring XBP1 binding on Vegfa, Il-4, and Il-6 segments. Bars represent mean ± SD of three experimental replicates. *P < 0.05, **P < 0.01, ***P < 0.001; t-test. e Expression of VEGFA, IL-4, and IL-6 versus XBP1s in all hTAM samples from CRC patients (n = 27). r, Spearman’s rank correlation test. f Kaplan–Meier survival curves for CRC patients with or without high expression levels of VEGFA, IL-4 and IL-6 in the GEO online database (GSE38832). The optimal survival cut point was determined via X-Tile statistical software
