Fig. 7

FNC protects the function of the thymus in the virally infected RM monkeys. The droplet-based scRNA-seq (10× Genomics) was performed to characterize the immunological features in the thymus of the SARS-CoV-2(+) RM monkeys, treated or untreated with FNC. a The top 30 enriched Gene Ontology (GO) biological process terms in the subset of CD4, CD8, and monocyte are shown. GO terms are labeled with name and ID, and the red-to-blue color indicates the level of p values. Interesting terms are labeled in green. b Histogram of expression levels of the GO biological process terms, including T-cell activation, T-cell activation involved in immune response, innate immune response, and positive regulation of immune system process, in each subset cells from the infected RM monkeys, treated or untreated with FNC. Horizontal lines represent median values, and the statistical analysis is shown at the top of each figure. Light blue box: untreated (#17041); pink box: FNC treated (#17368). c Histogram of expression levels of GO biological process terms related to response to the virus in each cell subset. Horizontal lines represent median values, and the statistical analysis is shown at the top of each figure. Blue box: untreated (#17041); green box: FNC treated (#17368). d Histogram of expression levels of GO biological process terms related to IL4, IL10, IL13 biosynthesis, and Th17 lineage commitment in each subset cells. Horizontal lines represent median values, and the statistical analysis is shown at the top of each figure. Blue box: untreated (#17041); green box: FNC treated (#17368). e Representative images of immunofluorescent staining of RORγt, IL10, IL13, and IL4 in thymus samples. The regions of interest (ROI) are boxed in white (up), and their magnified photos are shown below. Scale bars, 100 µm (up) and 20 µm (down). p Value was analyzed by Mann–Whitney U test