Fig. 4 | Signal Transduction and Targeted Therapy

Fig. 4

From: Single-cell transcriptomic profiling unravels the adenoma-initiation role of protein tyrosine kinases during colorectal tumorigenesis

Fig. 4The alternative text for this image may have been generated using AI.

Intermediate stages of epithelial cells among the physiological, benign, and malignant stages. a Signature scores for cells from physiological, benign, and mixed clusters (9_Mix4) in P1. Columns represent cells (n = 2420) annotated with cell type and tissue. Colors represent Z scores. b Log-normalized expression levels of key marker genes of intestinal stem and base crypt cells (LGR5, SOX9, ASCL2, OLFM4). Red cycle indicates cells of cluster 9_Mix4 (top, P1, n = 73) and cluster 9_Mix2 (bottom, P2, n = 87). c Single-cell trajectory of 554 physiological epithelial cells, 73 adenoma-precursor cells (cluster 9_Mix4), and 1793 benign epithelial cells. The trajectory was constructed using monocle according to gene expression. Each dot represents a single cell, and colors represent cell types (left) and the pseudotime of trajectory (right). d Heatmap of differentially expressed genes among normal, adenoma precursor (cluster 9_Mix4), and adenoma epithelial cells. Genes related to carcinogenesis are highlighted. e Representative images of BMX and SH2D6 IHC staining of normal tissue (n = 20) and adenoma (n = 20). Black arrows indicate positively stained cells. Scale bar, 200 μm. f Venn diagrams show the overlap of upregulated genes in P1 epithelial mixed clusters (cluster 4, 5, and 9) and P2 epithelial mixed clusters (cluster 7 and 9)

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