Fig. 8

Single-cell analysis of AngII-induced AAA with DMSO or inhibitor. a T-SNE plot showing the clustering of 22, 975 cells isolated from aortic tissues of three groups by split view. The dot plot showed that the average scaled expression levels of marker genes were consistent with the identification of the first five samples. AA, healthy abdominal aorta; D_A, AngII-induced AAA with DMSO; IN_A, AngII-induced AAA with inhibitor. b The bar chart showed the number of major cell types across the three groups. c The line chart showed the proportion of major cell types across the three groups. The ratio of VSMCs and immune cells changed the most. d The fold change of all genes with a significant difference in VSMCs between D_A and IN_A groups was presented by the volcano plot. e KEGG analysis of downregulated genes in IN_A vs. D_A group. f Identification of VSMC subtypes based on the previous marker genes in the first five samples. g The bar chart showed the ratio of five VSMC subtypes across three groups, in which VSMC 1 and VSMC 4 changed obviously. h In addition to the existing marker genes, the top differentially expressed genes (DEGs) in each subtype were shown by the heatmap. i The expression level changes of Malat1 in each subtype across three groups are shown by the violin plot, in which the Malat1 expression level of VSMC 4 increased in the D_A group